| 洪辉华,蔡宛如.芪冬活血饮对急性肺损伤大鼠caveolin-1和细胞因子的影响[J].浙江中西医结合杂志,2015,25(5): |
| 芪冬活血饮对急性肺损伤大鼠caveolin-1和细胞因子的影响 |
| The effects of Qidong Huoxue Decoction on caveolin-1 and cytokines in acute lung injury rats |
| 投稿时间：2014-11-05 修订日期：2015-03-18 |
| DOI： |
| 中文关键词: 芪冬活血饮,急性肺损伤,小窝蛋白-1,细胞因子 |
| 英文关键词:Qidong Huoxue Decoction,acute lung injury, Caveolin-1, cytokine. |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 摘 要 目的 观察芪冬活血饮对脂多糖所致大鼠急性肺损伤（ALI）小窝蛋白-1及细胞因子的影响。方法 将50只SD大鼠随机分为空白对照组、模型组、芪冬活血高、中、低组，每组10只。通过气道内滴注脂多糖建立大鼠急性肺损伤模型。芪冬活血高、中、低剂量组造模前24、12h及造模后12h分别以16、8、4mL/kg芪冬活血饮灌胃，空白组及模型组以8mL/kg生理盐水灌胃。各组大鼠均在造模后24h处死，收集标本。检测肺泡灌洗液细胞因子变化；常规HE染色，光镜下观察肺组织病理变化；免疫组化法检测肺组织Cav-1表达；RT-PCR检测Cav-1mRNA表达。结果 1.芪冬活血饮可以减少肺损伤大鼠肺泡结构破坏，肺水肿及炎性细胞浸润。2.空白组TNF-?、IL-1β、IL-10含量最低，与其余各组比较差异均有统计学意义（P均＜0.01）。模型组TNF-?、IL-1β含量均高于中、高剂量组[（52.59±12.78）比（39.87± 8.14）和（27.78±10.99）pg/mL，（42.39±11.15）比（33.83±8.38）和（24.90±7.06）pg/mL，P＜0.05或P＜0.01]，IL-10含量低于中、高剂量组[(15.63±2.13）比（20.98±2.17）和（22.33±2.31）pg/mL，P＜0.05或P＜0.01]。中、高剂量组比较TNF-?、IL-1β 差异P均<0.05。3.Cav-1免疫组化积分及mRNA表达量均为空白对照组最低，模型组最高。除模型组与低剂量组免疫组化积分（8.58±1.39比7.33±1.16）比较差异无统计学意义，空白组、模型组与各中药比较P均＜0.01；Cav-1免疫组化积分及mRNA相对表达高剂量组低于低剂量组 [（5.91±1.11比7.33±1.16）和（35.27±3.31比62.34±5.66），P＜0.01]。结论 芪冬活血饮对LPS诱导的大鼠ALI有保护作用，其机制可能与抑制Caveolin-1表达，降低促炎细胞因子TNF-?、IL-1β水平，升高抗炎细胞因子IL-10水平，纠正炎症失衡有关。 |
| 英文摘要: |
| Abstrct:Objectives:To observe the effects of qidonghuoxue decoction (QD) on caveolin-1and cytokines in rats with acute lung injury induced by LPS,and to explore the protective mechanism.Methods:50 SD rats were divided into five groups randomly: control group、model group、high-dose QD group、medium-dose QD group、low-dose QD group. The acute lung injury model was established by intratracheal instillation of lipopolysaccharide. QD was administrated via esophagus to rats in High, medium,low QD groups 24hs、12hs before modeling, and 12h after modeling with dose 16、8、4ml/kg respectively;NS was used insteadly in control and model groups with dose 8ml/kg. All the rats were executed 24hs after modeling. Results:1.HE staining showed that QD can reduce the damage to rat pulmonary alveolar structure, pulmonary edema and inflammatory cells infiltration.2. The level of TNF-?、IL-1β、IL-10 was lowest in control group,compared to other groups differences were statistically significant (P<0.01). The level of TNF-?、IL-1β、IL-10 was lowest in control group,compared to other groups differences were statistically significant (P<0.01).The level of TNF-?、IL-1β in model group were significantly higher than those of high/meidium dose group[（52.59±12.78）vs（39.87± 8.14）and（27.78±10.99）pg/mL，（42.39±11.15）vs（33.83±8.38）and（24.90±7.06）pg/mL，P＜0.05 or P＜0.01],while IL-10 was opposite[(15.63±2.13）vs（20.98±2.17）and（22.33±2.31）pg/mL，P＜0.05 or P＜0.01].There were significant difference between the high-dose group and medium-dose group of TNF-? and IL-1β(P＜0.05).3.The immunohistochemistry score(IHC) and the mRNA relatively expression of Cav-1 in control group was the lowest, while the model group was the highest.There were significant differences between control/model group and QD groups（p<0.01）except difference between model group and low-dose group in IHC;The IHC and mRNA expression of Cav-1 of high-dose were significantly lower than those of low-dose group[(5.91±1.11VS7.33±1.16)﹠(35.27±3.31 VS 62.34±5.66），P＜0.01].Conclusions: QD has a protective effect on ALI rats induced by LPS,cause it could inhibit Caveolin-1, increased IL-1 β,TNF- ? and decreased IL-10,and it’s kind of dose related. |
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