| 陶晓薇,毛其芬.槲皮素下调HSP27蛋白的表达诱导人胶质母细胞瘤T98G凋亡[J].浙江中西医结合杂志,2015,25(5): |
| 槲皮素下调HSP27蛋白的表达诱导人胶质母细胞瘤T98G凋亡 |
| Quercetin down-regulates the expression of HSP27 inducing the apoptosis in glioblastoma cell line T98G Tao Xiaowei1, Mao Qifeng2. 1Gongshu district Xiaohe Hushu street community health center (310011). China 2Tongde hospital of Zhejiang province(310012). China |
| 投稿时间：2014-10-23 修订日期：2014-12-25 |
| DOI： |
| 中文关键词: 槲皮素 T98G HSP27 凋亡 caspase-3 |
| 英文关键词:quercetin T98G HSP27 apoptosis caspase-3 |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨天然药物槲皮素对人胶质母细胞瘤的生物效应及机制。方法：将人胶质母细胞瘤细胞系T98G用槲皮素治疗,采用MTT法检测治疗后T98G细胞的增殖抑制情况；通过流式细胞术检测槲皮素治疗后,T98G细胞的凋亡情况并用western blot方法检测细胞活化caspase-3 (cleaved caspase-3)的表达和热休克蛋白27(HSP27)的表达；将T98G细胞的HSP27基因用特异性HSP27 siRNA沉默后再用槲皮素治疗,检测槲皮素对T98G细胞的增殖抑制作用和凋亡诱导效应。结果：槲皮素显著抑制T98G细胞的增殖且呈剂量和时间依赖性；槲皮素可诱导T98G细胞的凋亡和caspase-3的活化；槲皮素治疗后,T98G细胞的HSP27表达显著下降,当用HSP27 siRNA转染T98G细胞后, 槲皮素对T98G细胞的凋亡诱导活性显著提高。结论： 槲皮素有良好的抗神经胶质母细胞瘤的生物活性,其抗肿瘤效应的机制可能和下调HSP27蛋白有关。 |
| 英文摘要: |
| objective: To explore the effect and mechanism of natural medicine quercetin in glioblastoma cells. Methods: T98G cells were treated with quercetin and the proliferation was measured using the MTT assay. The apoptosis of T98G was measured by flow cytometry and the expression of cleaved caspase-3 and HSP27 was detected by western blot in T98G cells treated with quercetin. The specific siRNA transfected method was used for blocking of the expression of HSP27 gene before the T98G cells were treated with quercetin, and then the proliferation and apoptosis were detected. Results: Quercetin significantly inhibited the proliferation of T98G cells inducing apoptosis and activation of caspase-3. Quercetin treatment significantly decreased the expression of HSP27. Furthermore, gene silence of HSP27 enhanced the anti-tumor effect of quercetin. Conclusion: Quercetin induced apoptosis of T98G cells via down-regulating the expression of HSP27.. |
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