| 沈双,卢振产,胡正刚,王亚仙.PPARγ C161T基因多态性的表达与进展性脑梗死的相关性研究[J].浙江中西医结合杂志,2014,24(10): |
| PPARγ C161T基因多态性的表达与进展性脑梗死的相关性研究 |
| The PPARγ expression of C161T polymorphism associated with progressive ischemic strokeShen Shuang,Lu Zhen-chan, Wang Ya-xian, Hu Zhen-gang |
| 投稿时间：2014-04-09 修订日期：2014-07-24 |
| DOI： |
| 中文关键词: PPARγ基因 进展性脑梗死 单核苷酸多态性( SNP) |
| 英文关键词:peroxisome proliferator-activated receptor gamma(PPARγ) gene Progressive ischemic stroke Single nucleotide polymorphism (SNP) |
| 基金项目:湖州市科技计划项目(2012YS16) |
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| 中文摘要: |
| 目的 探讨PPARγ基因多态性及其表达与进展性脑梗死的关系。方法 本研究共纳入450例患者: 将225例进展性脑梗死患者纳入进展组,并选择与进展性脑梗死患者年龄、性别、既往疾病史相似的同期住院的非进展性脑梗死患者225例为非进展组。应用多聚酶链-限制性片段长度多态性( PCR-RFLP)技术检测PPARγ基因C161T的基因型,酶联免疫吸附(ELISA)法检测PPARγ基因表达水平。结果：在进展性和非进展性脑梗死患者中PPARγ的C161T基因多态性频率无明显差异(p>0.5)。在入院时、入院后72小时以及发病后4周,携带C等位基因脑梗死患者的PPARγ表达量低于携带T基因的脑梗死患者(p<0.01),并且在携带C等位基因患者中进展性脑梗死患者的PPARγ表达量低于非进展性梗死患者(p<0.01)。与入院时比较,入院后72小时以及发病后4周各基因型的PPARγ表达量明显升高(p<0.01)。结论 PPARγC161T基因多态性的表达可能与进展性脑梗死的发生有关。 |
| 英文摘要: |
| Objective To investigate the relationship between peroxisome proliferator-activated receptor gamma (PPARγ) expression of C161T polymorphism and progressive ischemic stroke. Methods The study included two hundred and twenty-five progressive ischemic stroke patients, who were classified as progressive ischemic stroke group (n=225). And two hundred and twenty-five non-progressive ischemic stroke patients, whose age, gender, past history of disease, were similar with progressive ischemic stroke patients, were classified non-progressive ischemic stroke group(n=225). The C161T variant was examined using polymerase chain reaction restriction fragment length polymorphism(PCR - RFLP)．The serum PPARγ was measured by ELISA. Results There were no statistical differences in frequencies of genotype and allele between the progressive and non-progressive ischemic stroke(p>0.5). At admission, 72 hours after admission and four weeks after onset, the C allele carriers had lower levels of PPARγ, compare with the C allele carriers, in all of patients(p<0.01). And the PPARγ expression in progressive ischemic stroke patients significantly lowers than in non- progressive ones,who were with the C allele carriers(p<0.01). The PPARγ expression significantly increased in each genotype , Compared with in admission,in 72 hours after admission and four weeks after the onset(p<0.01). Conclusions The PPARγ expression of C161T polymorphism was probably related to progressive ischemic stroke. |
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