| 侯兆阳,陈哲.羟基红花黄色素A在急性脊髓损伤中对细胞凋亡的影响[J].浙江中西医结合杂志,2014,24(9): |
| 羟基红花黄色素A在急性脊髓损伤中对细胞凋亡的影响 |
| Effects of hydroxysafflor yellow A on neural cell apoptosis after acute spinal cord injury |
| 投稿时间：2014-03-15 修订日期：2014-07-11 |
| DOI： |
| 中文关键词: 脊髓损伤 HSYA 细胞凋亡 |
| 英文关键词:Spinal cord injury HSYA Apoptosis |
| 基金项目:浙江省教育厅科研项目(Y200906743) |
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| 中文摘要: |
| 目的：观察羟基红花黄色素A(HSYA) 在急性脊髓损伤（ASCI）中的治疗作用及潜在机制。方法：SD 大鼠随机分为空白对照组、损伤对照组和HSYA治疗组，采用ALLEN’S打击法建立急性脊髓损伤模型，各组分别于术后1、7、14d观察其行为学评分，14d通过免疫组化的方法观察脊髓损伤部位神经细胞凋亡(caspase-3p20、Bcl-2与Bax)。结果：（1）行为学观察评分：HSYA治疗组与损伤对照组下肢功能均有恢复(P＜0.05），且HSYA治疗组恢复更明显(P＜0.05）；（2）免疫组化检测：caspsase-3阳性细胞数HSYA治疗组表达较损伤对照组少(P＜0.05）；Bcl-2阳性细胞HSYA治疗组的表达较损伤对照组多(P＜0.05）；Bax阳性的细胞HSYA治疗组的表达较损伤对照组少(P＜0.05）。结论：HSYA在急性脊髓损伤中具有抑制神经细胞凋亡，减少了脊髓损伤部位的继发性损伤，从而促进功能的恢复的作用，因此HSYA有可能成为治疗脊髓损伤的药物之一。 |
| 英文摘要: |
| Objective：The aim of this study was to determine the therapeutic efficacy of hydroxysafflor(HSYA) treatment after acute spinal cord injury(ASCI) in rat and to investigate the underlying mechanism . Methods：ASCI was induced using the modified weight-drop method in Sprague–Dawley rats. The SCI animals were randomly divided into three groups: sham group (laminectomy only); vehicletreated group , and additionally with HSYA-treated group . Locomotors functional recovery was assessed during the 1、7、14 days post operation period by performing open-field locomotors tests . At the end of the study , the segments of spinal cord encompassing the injury site were removed for histopathological analysis. Immunohistochemistry was performed to observe the expression of B-cell CLL/lymphoma-2 (Bcl-2), BCL-2-associated X protein (Bax) and caspase-3 .Results：The HSYA-treated animals had significantly better locomotor function recovery than the vehicletreated group(P＜0.05). In addition , Compared with the vehicletreated group,the HSYA-treated significantly had increased expression of Bcl-2(P＜0.05), reduced expression of caspase-3(P＜0.05) and Bax (P＜0.05) after spinal cord injury. Conclusion：These findings suggest that HSYA treatment after SCI can significantly improve locomotor recovery, and this neuroprotective effect may be related to the inhibition of neural apoptosis . Therefore , HSYA may be useful as a promising therapeutic agent for SCI. |
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