| 官佳佳,陈飞,吴伽勒,胡申江,牟芸.芪苈强心改善糖尿病心力衰竭大鼠左心室功能及血管内皮功能[J].浙江中西医结合杂志,2014,24(8): |
| 芪苈强心改善糖尿病心力衰竭大鼠左心室功能及血管内皮功能 |
| Improvement of left ventricular function and vascular endotheial function by Qili qiangxin in diabetes rats with heart dysfunction |
| 投稿时间：2013-12-23 修订日期：2013-12-24 |
| DOI： |
| 中文关键词: 芪苈强心 糖尿病 心力衰竭 左心室功能 血管内皮功能 |
| 英文关键词:qiliqiangxin diabetes mellitus (DM) herat faiule left ventricular function vascular endothelial relaxation function |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 目的：以糖尿病大鼠作为实验模型，观察芪苈强心改善糖尿病大鼠心功能的同时，能否改善其血管内皮功能。
方法：选用Sprague-Dawley（SD）雄性大鼠随机分为3组：对照组、糖尿病组（DM）和芪苈强心组。DM组和芪苈强心组大鼠腹腔注射链脲佐菌素（STZ）诱导糖尿病模型，造模成功后（随机血糖>16.7mmol/L）即开始分别给予生理盐水和芪苈强心灌胃，对照组给予生理盐水灌胃。灌胃8周后各组大鼠测定体重，超声心动图检查测左室舒张末期内径(LVIDD)、左室收缩末期内径（LVIDS）、射血分数（EF）、左室短轴缩短率（FS），血流动力学检查测左室收缩峰压（LVESP）、左室舒张末压（LVEDP）、左室内压最大上升( dp/dtmax)和下降速率(-dp/dtmax)，并测心脏重量指数。分离胸主动脉，以血管环技术观察离体胸主动脉环对内皮依赖性扩血管物质乙酰胆碱的舒张反应，以之代表大鼠血管内皮功能。
结果：1）一般情况：与对照组比，糖尿病组大鼠体重明显减轻（221±46 vs 421±62 g， p<0.01），心肌重量/体重比明显增高（4.07±0.67 vs 3.23±0.14 mg/g，p<0.01），心率变慢（350±54 vs 432±44次/每分，p<0.01）。与糖尿病组比，芪苈强心组大鼠体重、心肌重量/体重比没有改善，心率增高（368±60 vs 350±54次/每分，p<0.01）。2）心脏超声检测：与正常组比，糖尿病组LVIDD明显减小（6.79±0.58 vs 7.73±1.19 mm，p<0.05），EF，FS降低分别为 37.15±8.81 vs 52.04±9.68%, p<0.01 和 0.72±0.11 vs 0.86±0.09, p<0.01)；与糖尿病组比，芪苈强心组LVIDD无明显变化，但LVIDS明显减小（3.82±0.66 vs 4.25±0.53, p<0.05），EF，FS增高（分别为43.31±6.87 vs 37.15±8.81% , p<0.05和 0.81±0.07 vs 0.72±0.11, p<0.01）。3）血流动力学检测：与正常对照组比，糖尿病组 dp/dt明显下降（4044.66±1303.27 vs 6015.63±905.70，p<0.01 ），LVSP、LVEDP、-dp/dtmax无显著性差异；与DM组相比，芪苈强心组 dp/dt明显增高(5338.33±946.37 vs 4044.66±1303.27，p<0.01)，LVSP、LVEDP、-dp/dtmax无显著性差异。4）胸主动脉环对乙酰胆碱的反应性测定：与正常组比，糖尿病组胸主动脉环对乙酰胆碱介导的舒张百分比降低，最大舒张百分比下降33%;与糖尿病组相比,芪苈强心组改善乙酰胆碱介导的舒张反应，最大舒张百分比增加12%,说明芪苈强心组大鼠血管内皮舒张功能得到改善。
结论：芪苈强心能改善糖尿病大鼠的血管内皮的舒张功能，这可能是其改善心力衰竭大鼠左室收缩功能的机理之一。 |
| 英文摘要: |
| Abstract
objective: To evaluate the effects of Chinese traditional medicine Qiliqiangxin in the treatment of diabetes rats with heart dysfunction by assessment of the left ventricular systolic, diastolic function and vascular endothelial function.
Method: Dibetes rats were gavaged with Qiliqiangxin for 8 weeks. We examined the left ventricular diastolic diameter (LVIDD), left ventricular systolic diameter (LVIDS), left ventricular eject fraction (EF) and fraction shortening (FS) by echocardiography. Left ventricular end systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), dp/dt max were evaluated by catheter. Vascular endothelial function was measured by the tension of isolated thoracic aorta with endothelium dependant acetylcholine response test. in the isolated thoracic aorta.select Sprague-Dawley (SD) random male rats were divided into 3 groups:control group, diabetes mellitus (DM) and qiliqiangxin group. the rats of DM group and qiliqiangxin group intraperitoneal injection of Streptozotocin (STZ)-induced diabetic model, after the model succesed (random blood sugar >16.7mmol/L)，then begin intragastric infusion with saline solution and effects of qiliqiangxin, the control group given saline solution. after filling stomach 8 week， the group big rat determination weigh，UCG check LVIDD， and LVIDS, and EF, and FS, blood flow dynamics check LVSP, and LVEDP, and DP/dtmax ，and -DP/dtmax， and heart weight index. Separation of aorta, vascular ring technology to observation of isolated thoracic aorta rings on diastolic dysfunction of the endothelium-dependent vasodilator substance acetylcholine response, according to the vascular endothelial function in rats.
Result: 1):General conditions: compared Compared with the normal control group, rat body weight significantly and heart rate reduced significantly in diabetes group (221 ± 46 vs 421 ± 62 g,p<0.01; 368 ± 60 vs 350 ± 54 times/min, p<0.01), heart weight/body weight ratio significantly increased significantly (4.07 ± 0.67 vs 3.23 ± 0.14 mg/g,p<0.01), slow heart rate (350 ± 54 vs 432 ± 44 times/min, p<0.01). Compared with the diabetes group, the qiliqiangxin group rats body weight, heart weight/body weight ratio has did not improved in Qiliqiangxin group, increased but heart rate increasedheart rate (368 ± 60 vs 350 ± 54 times/min, p<0.014). 2) ultrasound of the heartUltrasonography: Ccompared with the normal group, LVIDD significantly reduces in diabetes group (6.79±0.58 vs 7.73±1.19 mm，p<0.05± 1.19 ± 0.58 vs 6.79 mm,p<0.05), EF,FS lowerdecreased significantly in diabetes group (37.15±8.81 vs 52.04±9.68%, p<0.01; 0.72±0.11 vs 0.86±0.09, p<0.01), respectively to ± ± 8.81 vs 37.15 9.68%, p<0.01 and 0.72 ± 0.11 vs 0.86 ± 0.09, p<0.01); compared with the diabetes group, LVIDD in Qiliqiangxin group did not changed significantly, but LVIDS decreased significantly (3.82±0.66 vs 4.25±0.53, p<0.05),EF, FS increased significantly (43.31±6.87 vs 37.15±8.81% , p<0.05; 0.81±0.07 vs 0.72±0.11, p<0.01).effects of qiliqiangxin LVIDD no apparent change in the group, But LVIDS significantly reduce (3.82 ± 0.66 vs 4.25 ± 0.53, p<0.05), high EF,FS (± 8.81% ± 6.87 vs 43.31, respectively, p<0.05 and 0.81 ± 0.07 vs 0.72 ± 0.11, p<0.01). 3) hemodynamic Hemodynamic analysis: Compared with normal control group, dp/dt max decreased significanltly in the diabetic Group (4044.66±1303.27 vs 6015.63±905.70，p<0.01), LVSP and LVEDP and the -dp/dtmax without significant difference; compared with DM group, dp/dt max significantly increased in Qiliqiangxin Group (5338.33±946.37 vs 4044.66±1303.27，p<0.01), LVSP and , LVEDP and the-DP/dtmax were without significant differences. 4) Thoracic aorta response to the acetylcholine: compared with normal group, acetylcholine-mediated vasodilation rate decreaed in diabetes group, the maximum rate decreased 33%; compared with diabetes group, the maximum vasodilation rate increased 12% in Qiliqiangxin group. It represented the improvement of endothelial function in Qiliqiangxin group.aortic ring responses to acetylcholine of determination: compared with the normal group, groups of thoracic aortic rings of diabetes on concentration of acetylcholine-mediated vasodilation is lower as a percentage, percentage of maximum diastolic drop 33%; compared with diabetes, effects of qiliqiangxin group improved concentration of acetylcholine-mediated vasodilation response, increase in percentage of maximum diastolic 12%.
Conclusion: Qiliqiangxin improved vascular endothelial relaxation function in diabetic rats. This might be one of the mechanisms that causes the restoration of left ventricular systolic and diastolic function in rats with heart failure |
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