| 韩明训,赵乌兰,王一鸣,王永华,沈晓丽.针刺及川芎嗪对急性药物性聋豚鼠
耳蜗内氧自由基生成影响实验研究[J].浙江中西医结合杂志,2013,23(10):782-784 |
| 针刺及川芎嗪对急性药物性聋豚鼠
耳蜗内氧自由基生成影响实验研究 |
| Effects of Tetramethylpyrazine and Acupuncture on Cochlear Free Radical Expression of Piunea Pigs with AcuteDrug -induced Deafness |
| 投稿时间：2013-07-02 |
| DOI： |
| 中文关键词: 豚鼠 药物性聋 川芎嗪 针刺 氧自由基 |
| 英文关键词:piunea pigs drug-induced deafness tetramethylpyrazine acupuncture free radical formation |
| 基金项目:基金项目: 国家自然基金面上项目（No.81173309）；浙江省中医药科技计划项目（No.2010ZA015） |
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| 中文摘要: |
| 目的：探讨川芎嗪（TMP）、针刺单独应用及联合使用对庆大霉素（GM）致聋豚鼠耳蜗内氧
自由基生成的影响。方法：50 只健康豚鼠随机分为正常组、模型组、川芎嗪组、针刺组及TMP+针刺
组，每组10 只，正常组按2.5mL/（kg·d）剂量腹腔注射生理盐水，其余各组腹腔注射庆大霉素
100mg/（kg·d），连续14 天，TMP组、针刺组、TMP+针刺组同时灌服TMP和（或）针刺相关穴位。造模
结束后迅速断头处死，取耳蜗标本。正常组与模型组动物于用药前及停药后第1 天分别测试听觉脑
干诱发电位（ABR）以判断造模是否成功；硫代巴比妥法检测血清丙二醛（MDA），黄嘌呤氧化酶法
测定超氧化物歧化酶（SOD）。结果：各治疗组与模型组比较MDA 表达均明显减少（P<0.01），尤以
TMP+针刺组改善明显（P<0.01）。各治疗组SOD活力均有提高，各组间差异无统计学意义（P>0.05）。
结论：针灸配合TMP明显提高耳蜗组织内SOD 活力，防止脂质过氧化，对急性耳毒性药物早期损
伤具有一定的防治作用。 |
| 英文摘要: |
| Objective：To investigate the effect of acupuncture and tetramethylpyrazine（TMP）on cochlear free radical
expression of mice with acute drug-induced deafness. Methods：Fifty guinea pigs were randomly divided into 5
groups，including normal，model，TMP alone，acupuncture alone，and TMP plus acupuncture groups，10 animals in
each group. Each group received different procedures. The normal group was prepared by consecutively intraperi-
toneal injection with normal saline of 2.5 mL/（kg·d）. Acute drug-induced deafness was made by consecutively in
traperitoneal injection with gentamycin（100 mg/kg·d）in other groups. All groups were injected for consecutive 14
days. Before and 1 day after 14-day injection, the mice were tested ABR to identify whether the models were suc-
cessful. The methods of thiobarbituric acid and xanthine oxidase were used to test serum malondialdehyde（MDA）
and superoxide dismutase（T-SOD）. Results：The expression of MDA was reduced in all treatment groups as com-
pared with that of the model group（P<0.01），with the most reduction seen in the TMP plus acupuncture group. The
activity of SOD was enhanced in all treatment group，but with no significant difference among the treatment groups
（P>0.05）. Conclusion：TMP plus acupuncture can effectively increase the activity of SOD and reduce the expres-
sion of MDA in the cochlear tissue. They may have effect against gentamycin-induced inner ear diseases.
Key words piunea pigs drug-induced deafness tetramethylpyrazine acupuncture free radical formation |
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