| 董 刚,乐军,周 辉,项 东.PDTC 关节腔注射对兔创伤性OA 软骨MMP-1、MMP-9mRNA 表达的影响[J].浙江中西医结合杂志,2013,23(8):609-612 |
| PDTC 关节腔注射对兔创伤性OA 软骨MMP-1、MMP-9mRNA 表达的影响 |
| Effects of Intra-articular Injection of Pyrrolidine Dithiocarbamate on mRNA expressions of MMP-1 and MMP-9in the Cartilage of Rabbits with traumatic osteoarthritis |
| 投稿时间：2013-04-16 |
| DOI： |
| 中文关键词: 兔 骨关节炎 核因子-资B 基质金属蛋白酶-1 基质金属蛋白酶-9
吡咯烷二硫代氨基甲酸酯 |
| 英文关键词:rabbits osteoarthritis metalloproteinase-1 metalloproteinase-9 pyrrolidine dithiocarbamate |
| 基金项目:基金项目：杭州市医药卫生科技计划项目（No.2009A026） |
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| 中文摘要: |
| 目的：观察吡咯烷二硫代氨基甲酸酯（PDTC）关节腔注射对兔创伤性骨关节炎（os-
teoarthritis，OA）软骨中基质金属蛋白酶-1（MMP-1）、基质金属蛋白酶-9（MMP-9）mRNA 表达及
NF-kBp65活性的影响，探讨PDTC 对兔创伤性OA 软骨退变进程的影响。方法：取20 只新西兰兔
行右膝横断前交叉韧带术（ACLT），术后1 周随机分为对照组和PDTC 组，每组10 只。PDTC 组关节
腔注射PDTC2mg/（只·次），每周2 次，连续8 周，对照组同一时间点关节腔注射等容量生理盐水。
末次用药3天后处死白兔，解剖显微镜下观察股骨内髁软骨的形态学变化，逆转录聚合酶链反应
（RT-PCR）检测股骨内髁软骨中MMP-1、MMP-9mRNA 表达量，免疫组化检测股骨内髁软骨中
NF-可Bp65 蛋白表达。结果：形态学示PDTC 组软骨退变明显轻于对照组（P<0.05），RT-PCR 示
PDTC 组MMP-1、MMP-9mRNA表达量均低于对照组（P<0.01），免疫组化示PDTC 组NF-kBp65 活
性系数明显低于对照组（P<0.01）。结论：PDTC关节腔注射可能通过阻断创伤性OA 软骨中NF-kB
激活，进而抑制MMP-1、MMP-9mRNA表达，发挥软骨保护作用。 |
| 英文摘要: |
| Objective：To observe the effects of intra-articular injection of pyrrolidine dithiocarbamate（PDTC）on mR-
NA expressions of metalloproteinase-1，-9（MMP-1 and MMP-9）and the activities of nuclear factor-kappaB p65
（NF-kBp65）in the articular cartilage of rabbits with traumatic osteoarthritis（OA），and to explore the influence of
PDTC on cartilage degeneration in traumatic OA. Methods：Twenty New Zealand rabbits underwent anterior cruciate
ligament transaction（ACLT）of the right knee and then were divided into two groups. The animals in the PDTC
group were injected with PDTC（2mg）in the knees after operation, twice a week for 8 weeks. Rabbits in the control
group were treated with normal saline under the same condition. All rabbits were sacrificed in 3 days after the last
treatment. Cartilagineus changes of the medial femoral condyles were grossly checked and OA severity was observed
under dissecting microscope. The mRNA expressions of MMP-1 and MMP-9 in the cartilage of the medial femoral
condyles were assessed by reverse transcription-polymerase chain reaction （RT-PCR）. Immunohistochemical and
morphometric analyses were adopted to detect the protein expression of NF-kBp65 in the cartilage. Results: Carti-
lage degeneration in the PDTC group was significantly slighter than that in the control group（P<0.05）. The mRNA
expressions of MMP-1 and MMP-9 in the PDTC group were significantly lower than those in the control group（P<
0.01）. The activity coefficient of NF-kBp65 in the PDTC group was significantly lower than that in the control
group（P<0.01）. Conclusion：The intra-articular injection of PDTC can inhibit the mRNA expressions of MMP-1 and
MMP-9 by blocking the activities of NF-kB in the cartilage of rabbits with traumatic OA. PDTC may have a pro-
tective effect on articular cartilage in traumatic OA. |
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