| 杨国良,张学进,胡丹丹.肺积方调节CD4+CD25+Treg 细胞及T 淋巴细胞增殖与抗Lewis 肺癌移植鼠肿瘤生长作用研究[J].浙江中西医结合杂志,2013,23(8):605-612 |
| 肺积方调节CD4+CD25+Treg 细胞及T 淋巴细胞增殖与抗Lewis 肺癌移植鼠肿瘤生长作用研究 |
| Effects of Feijifang on Levels of CD4+CD25+Treg and T Lymphocytes and Tumor Growth in Mice with Trans-planted Lewis Lung Cancer |
| 投稿时间：2013-02-05 |
| DOI： |
| 中文关键词: 肺癌移植 小鼠 肺积方CD4+CD25+Treg T淋巴细胞增殖指数 |
| 英文关键词:mice lung cancer transplantation Feijifang CD4+CD25+ regulatory T cells |
| 基金项目:基金项目：浙江省中医药科技计划项目（No.2010ZA097） |
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| 中文摘要: |
| 目的：观察肺积方联合化疗对Lewis肺癌移植小鼠肿瘤生长情况及脾脏CD4+CD25+Treg
细胞、胸腺T 淋巴细胞增殖变化的影响，探讨肺积方联合化疗抗肺癌免疫逃逸的作用机制。方法：
采用随机对照研究方法，将64 只Lewis肺癌移植小鼠随机分为对照组、肺积方组、综合组（肺积方
联合顺铂）、顺铂（DDP）组，每组16 只，并分别以生理盐水、肺积方、肺积方联合DDP、DDP 药物干
预，观察小鼠瘤体变化，流式细胞术检测小鼠脾脏CD4+CD25+Treg细胞含量，MTT法检测小鼠胸腺
和脾脏T 淋巴细胞增殖情况。结果：DDP 组、肺积方组、综合组小鼠瘤体、瘤重均小于对照组（P<
0.05），综合组瘤体、瘤重均小于DDP组和肺积方组（P<0.01）；第10、21 天DDP 组、肺积方组、综合
组小鼠胸腺、脾脏T淋巴细胞增殖指数与对照组比较，差异有统计学意义（P<0.05），其中肺积方组、
综合组T淋巴细胞增殖指数增高，而DDP 组T淋巴细胞增殖指数降低；DDP组干预后T 淋巴细胞
增殖指数明显低于干预前（P<0.05）；而肺积方组、综合组第21 天与第10 天T淋巴细胞增殖指数组
内比较无明显变化（P>0.05）。第10、21 天DDP组、肺积方组、综合组荷瘤小鼠胸腺CD4+CD25+Treg
细胞比例均较对照组降低（P<0.05）；DDP组第21 天胸腺CD4+CD25+Treg细胞比例较第10 天明显
升高（P<0.05）；综合组脾脏CD4+CD25+Treg细胞比例较低，与肺积方组、DDP组比较差异有统计学
意义（P<0.05）。结论：肺积方联合化疗具有抗Lewis 肺癌移植小鼠肿瘤生长的作用，该作用可能与
其下调CD4+CD25+Treg 细胞含量相关。 |
| 英文摘要: |
| Objective：To investigate the effect of Feijifang combined with chemotherapy on tumor growth and the
levels of CD4+CD25+ regulatory T cells（Treg）and T lymphocytes in mice with transplanted Lewis lung cancer and
the underlying mechanism of Feijifang modulating immune evasion. Methods：Sixty -four mice with transplanted
Lewis lung cancer were randomly divided into control group，Feiji group，comprehensive treatment group（Feijifang
combined with diamminedichloroplatinum），and diamminedichloroplatinum （DDP） group，16 mice in each. The
proliferation of T lymphocytes in the spleen and thymus was detected by MTT method and the percentage of
CD4+CD25+Treg was assessed by flow cytometry. The volume and weight of tumors were recorded and evaluated.
Results:The volume and weight of tumors in the DDP，Feiji，and comprehensive treatment groups were less than
those in the control group（P<0.05）. The volume and weight of tumors in the comprehensive treatment group were
less than those in the Feiji and DDP groups（P<0.01）；On day 10 and 21 after intervention, a significant difference
in the level of T lymphocytes was noted between the control group and the DDP, Feiji, and comprehensive treatment groups（P<0.05）；the level of T lymphocytes increased in the Feiji and comprehensive treatment groups but de-
creased in the DDP group（P<0.05）；and no significant difference was found between the level on day 10 and the
level on day 21 in the Feiji and comprehensive treatment groups（P>0.05）. On day 10 and 21，the percentage of
CD4+CD25+Treg was lower in the DDP，Feiji，and comprehensive treatment groups as compared with that in the
control group（P<0.05）；in the DDP group，the percentage of CD4+CD25+Treg increased after intervention as com-
pared with that before intervention（P<0.05）；and the percentage of CD4+CD25+Treg was lower in the comprehensive
treatment group than that in the DDP and Feiji groups（P<0.05）. Conclusion：FeiJifang combined with chemotherapy
can suppress the growth of Lewis lung cancer, which may be involved in decreasing the level of CD4+CD25+Treg. |
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