| 郭小芳,刘海波,任惠龙,龚维坤,成兴波.TNF-α和IL-1β对人脐静脉内皮细胞
蛋白C受体mRNA表达的影响[J].浙江中西医结合杂志,2013,23(2):98-101 |
| TNF-α和IL-1β对人脐静脉内皮细胞
蛋白C受体mRNA表达的影响 |
| Effects of TNF-α and IL-1β on EPCR mRNA in endothelial cells |
| 投稿时间：2012-09-24 |
| DOI： |
| 中文关键词: 内皮细胞
肿瘤坏死因子
白细胞介素-1
内皮细胞蛋白C受体 |
| 英文关键词:endothelial cells
tumor necrosis factor
interleukin-1
endothelial protein C receptor |
| 基金项目: |
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| 中文摘要: |
| 目的：观察肿瘤坏死因子α（TNF-α）和白细胞介素 1β（IL-1β）对人脐静脉内皮细胞
（ECV304）蛋白C受体（EPCR）mRNA表达的影响。方法：通过体外培养ECV304细胞，分别以含
TNF-α和IL-1β的培养基孵育ECV304细胞，行剂量和时间依赖性实验。采用实时定量聚合酶链反
应（quantitative real-time PCR ）技术测定 ECV304 细胞 EPCR mRNA 的表达。结果：TNF-α和
IL-1β均能显著下调 ECV304 EPCR mRNA 的表达，并呈剂量和时间依赖性（P<0.05）。结论：
TNF-α和IL-1β可能通过显著下调ECV304上EPCR mRNA的表达而成为损伤内皮细胞功能的一
个新作用机制。 |
| 英文摘要: |
| Objective：To investigate the effect of tumor necrosis factor alpha (TNF-α) and interleukin-1 beta
(IL-1β) on the expression of endothelial protein C receptor (EPCR) in human umbilical vein endothelial cells
(ECV304). Methods: Cultured ECV304 were used as target cells. Different concentrations of TNF-α or IL-1β
were added to the culture medium to establish the injury model of ECV304. EPCR mRNA were measured by
quantitative real-time PCR. Results: The expression of EPCR in ECV304 was down-regulated by TNF-α and
IL-1β in dose and time dependent manner. Conclusion: Both TNF-α and IL-1β may damage the function of
endothelial cells by down-regulating the expression of EPCR mRNA. |
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