| 陈筱凡.白花蛇舌草提取物体外抑制K562细胞增殖实验研究[J].浙江中西医结合杂志,2013,23(1):14-17 |
| 白花蛇舌草提取物体外抑制K562细胞增殖实验研究 |
| Hedyotis Herb Extracts Inhibited the Proliferation of Human Leukemia Cell Line K562 in vitro |
| 投稿时间：2012-08-31 |
| DOI： |
| 中文关键词: 白花蛇舌草提取物
K562细胞
跨膜电位
凋亡基因 |
| 英文关键词:hedyotis herb extracts
K562 cells
transmembrane potential
apoptosis genes |
| 基金项目: |
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| 摘要点击次数: 1063 |
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| 中文摘要: |
| 目的：观察白花蛇舌草提取物（HDE）体外对人白血病细胞株K562增殖的影响，探讨HDE
用于治疗白血病的作用机制。方法：以白血病K562细胞株为靶细胞，观察不同浓度的HDE对
K562细胞增殖的影响，及胞内线粒体跨膜电位（ΔΨm）的水平；检测低浓度HDE处理前后胞内Sur?
vivin、Bcl-2基因表达。结果：HDE（6.4mL/L）能明显抑制K562细胞增殖，且与浓度呈正相关（P<
0.05或P<0.01）。通过流式细胞术对碳氰化合物类亲脂性荧光染料（JC-1）检测发现，HDE能使靶
细胞内ΔΨm降低，且与HDE处理浓度呈负相关；K562细胞经低浓度HDE（3.2mL/L）处理3周后，
Survivin、Bcl-2基因表达均低于未经处理细胞的2.7倍和1.6倍。结论：HDE可能通过降低线粒体
跨膜电位，抑制抗凋亡基因的表达，抑制K562白血病细胞增殖。 |
| 英文摘要: |
| Objective: To determine the effect of hedyotis herb extracts (HDE) on the proliferation of human leuke?
mia cell line K562 in vitro. Methods: Human leukemia cell line K562 was used and was treated by varied con?
centrations of HDE(1.6,3.2,6.4,12.8,25.6ml/L). Variation of mitochondrial transmembrane potential was measured be?
fore and after treatment. Survivin and Bcl-2 in K562 cells were detected before and after treatment of 3.2ml/L
HDE for 3 weeks. Results: HDE at the dose of 6.4ml/L inhibited the proliferation of K562 cells and the effect
was enhanced as the dose increased. HDE decreased mitochondrial transmembrane potential in K562 cells and
the decrease was negatively related to the concentration of HDE. The expression of survivin and Bcl-2 on K562
cells before the treatment of 3.2ml/L HDE was 2.7 and 1.6 times higher than those after treatment. Conclusion:
HDE can inhibit the proliferation of K562 cells by decreasing mitochondrial transmembrane potential and sup?
pressing anti-apoptosis genes. |
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