| 年建华,陆逸庭,黄华平,周伟光,朱晓燕.姜黄素对大鼠肾缺血再灌注损伤及HIF-1α表达的影响[J].浙江中西医结合杂志,2012,22(12):943-945 |
| 姜黄素对大鼠肾缺血再灌注损伤及HIF-1α表达的影响 |
| Effects of Curcumin on Renal Ischemia-Reperfusion Injury and the Expression of HIF-1 Alpha in Rats |
| 投稿时间：2012-06-28 |
| DOI： |
| 中文关键词: 大鼠
肾缺血再灌注损伤
缺氧诱导因子-1α
姜黄素 |
| 英文关键词:rats
renal ischemia-reperfusion injury
hypoxia-inducible factor-1 Alpha
curcumin |
| 基金项目: |
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| 中文摘要: |
| 目的：观察姜黄素对肾缺血再灌注损伤及缺氧诱导因子-1α（HIF-1α）表达的影响。方法：
SD大鼠30只，随机分为假手术组、缺血再灌注组和姜黄素组，每组10只。姜黄素组术前连续3天
腹腔注射姜黄素200mg/kg，1天1次；缺血再灌注组术前3天则腹腔注射等容量生理盐水，1天1次，
假手术组模拟手术操作过程但不夹闭左肾动脉，另二组均予夹闭左肾动脉，并切除右肾。左肾夹
闭45min后松开动脉夹，2h后切除左肾置10%中性甲醛液固定，进行切片HE染色及免疫组化检测
各组肾组织HIF-1α表达水平；取左肾后立即通过下腔静脉采血，检测Scr、BUN含量。结果：与缺血
再灌注组比较，姜黄素组肾脏病理损伤明显减轻，血Scr、BUN水平及HIF-1α表达显著降低，差异有
统计学意义（P<0.05）。病理结果显示，缺血再灌注组有不同程度肾小球、肾间质充血，肾小管上皮
细胞水肿、细胞内玻璃样变及脂肪变性，肾小管腔内蛋白管型明显；姜黄素组仅个别标本存在蛋白
管型，水肿减轻。结论：姜黄素对肾缺血再灌注损伤具有干预作用，可能通过调节HIF-1α因子表
达，而发挥肾脏保护作用。 |
| 英文摘要: |
| Objective: To investigate the effects of curcumin(Cur) on renal ischemia-reperfusion injury(I/R) and
the expression of hypoxia-inducible factor-1 Alpha(HIF-1α) in rats. Method: Thirty Sprague-Dawley rats were
randomly divided into 3 groups, including a sham operation group, renal I/R group, and I/R+cur group. The I/R
and I/R+cur groups were subjected to left renal ischemia for 45 minutes and reperfusion for 2 h. The sham oper?
ation group underwent a surgical procedure that included isolation of the left renal artery without occlusion. In
cur treated group, the rats received intraperitoneal curcumin administration of 200 mg/kg for 3 consecutive days.
The sham operation group received equivalent volume of saline treatment for the same time. The left kidneys ere
taken out after 2-h reperfusion for measuring HIF-1α in renal tissue and serum specimens were immediately col?
lected from inferior vena cave for measuring creatinine and blood urea nitrogen(BUN). Results: Cur pretreatment
markedly reduced I/R-induced renal injury as indicated by histological alterations and the decreases in creati?
nine, BUN and HIF-1α (P<0.05). HE staining showed that hyperemia in glomcrulus and renal interstitium，edema
in renal tubular epithelial cells，hyalinization and steatosis in cells, and obvious protein cast in renal tubules in I/
R group, while that rare protein cast and the abatement of cellular edema in cur-treated group. Conclusion: Cur
may have a protective effect in renal injury induced by I/R. The protective effect of cur may be attributed to
the suppression of HIF-1α. |
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