| 吴海良,田静.气阴两虚型肺癌动物模型的建立和评价[J].浙江中西医结合杂志,2012,22(12):941-942 |
| 气阴两虚型肺癌动物模型的建立和评价 |
| Establishment and Evaluation of Lung Cancer Animal Model with Both Qi- and Yin-Deficiency in Tradi?tional Chinese Medicine |
| 投稿时间：2012-04-26 |
| DOI： |
| 中文关键词: 小鼠
肺癌
气阴两虚
动物模型 |
| 英文关键词:mice
lung cancer
Qi-and Yin-deficiency
animal model |
| 基金项目:山东省中医药科技发展计划项目（No.2009186） |
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| 摘要点击次数: 1140 |
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| 中文摘要: |
| 目的：建立评价一种类似气阴两虚型肺癌的动物模型。方法：采用“刨花烟熏”和灌胃温
热性中药制作气阴两虚模型，移植Lewis肺癌实体肿瘤，建立气阴两虚型肺癌实验动物模型，检测
血清IL-1、MDA水平，并应用金复康口服液进行反证该模型成立。结果：与对照组比较，模型组小
鼠血清IL-1、MDA水平显著增高（P<0.05，P<0.01）。经金复康口服液干预后，IL-1、MDA水平明显
下调（P<0.05）。结论：Lewie肺癌造模方法的基础上，采用“刨花烟熏”和灌胃温热性中药的方法来
建立肺癌气阴两虚病证结合的模型是可行的。 |
| 英文摘要: |
| Objective: To establish and evaluate a method for making animal model of lung cancer with both
Qi-and Yin-deficiency in traditional Chinese medicine(TCM) in mice. Methods: Thirty C57BL/6 mice were ran?
domly divided into control group, model group, and model+Jifukang group. In model and model+Jifukang group,
mice were fumigated with wood shavings and then fed with heat-property TCM decoction by gavage for deficient
Qi and Yin in terms of TCM theory. And then Lewis solid lung cancer was implanted to Qi-and Yin-deficient
mice. Jifukang oral liquid was administered at dose of 30g·kg-1·d-1in the model+Jifukang group. Levels of serum
IL-1 and malondialdehyde (MDA) were measured in the 3 groups. Results: Compared with the control group, lev?
els of serum IL-1 and MDA were increased in model group(P<0.05, P<0.01). The increase in IL-1 and MDA
was reversed by Jifukang in model+Jifukang group(P<0.05). Conclusion: Our experience indicated that both Qi-
and Yin-deficiency model could be achieved by wood shaving fumigating and gavage of heat-property decoction
based on Lewis mice model. |
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