| 罗培,周郁鸿.苦参碱对多发性骨髓瘤细胞的体外作用机制研究[J].浙江中西医结合杂志,2012,22(10):758-761 |
| 苦参碱对多发性骨髓瘤细胞的体外作用机制研究 |
| Effects of Matrine on Human Multiple Myeloma Cells In Vitro |
| 投稿时间：2012-05-16 |
| DOI： |
| 中文关键词: 多发性骨髓瘤 苦参碱 凋亡 |
| 英文关键词:multiple myeloma matrine apoptosis |
| 基金项目: |
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| 中文摘要: |
| 目的：阐明苦参碱对多发性骨髓瘤（MM）细胞的作用和内在机理。方法：MTT法检测苦参
碱对多发性骨髓瘤细胞株RPMI8226和多发性骨髓瘤原代细胞增殖的影响；根据中效原理分析苦
参碱联合亚砷酸对RPMI8226细胞株的联合作用；应用DAPI染色检测细胞凋亡。结果：M1TT比色
法检测结果显示，苦参碱对RPMI8226细胞及MM原代细胞均有生长抑制作用，与空白对照组比
较，差异有统计学意义（P<0.05）。体外联合亚砷酸作用RPMI8226细胞抑制率>50%时联合指数
（CI）<1。DAPI荧光染色，荧光显微镜下观察苦参碱以终浓度2.0g/L作用于RPMI8226细胞12h后
可见明显的凋亡小体出现。结论：苦参碱对RPMI8226细胞和MM原代细胞均有生长抑制作用，体
外联合亚砷酸作用MM细胞株RPMI8226细胞，抑制率>50%时呈协同作用。苦参碱通过诱导细胞
凋亡抑制RPMI8226细胞的增殖。 |
| 英文摘要: |
| Objective: To explore the effects of Matrine on human multiple myeloma (MM) cells and the underly?
ing mechanism. Methods: MTT assay was used to examine the effects of Matrine on the growth of MM primary
cells and MM cell line RPMI8226. Median-effect analysis was used to determine interactions between Matrine
and arsenious acid(ATO). Cell apoptosis were observed by fluorescence staining with DAPI. Results: MTT analy?
sis indicated that the proliferation of MM primary cells and RPMI8226 were significantly inhibited by Matrine in
comparison with the blank control group(P<0.05).The combination index (CI) was less than 1 when the combining
domino effect of Matrine and ATO was more than 50%. Apoptotic body of RPMI8226 was evidently seen by fluo?
rescence microscope after Matrine treatment (final concentration: 2.0g/L). Conclusion: Matrine inhibited the
growth of MM primary cells and RPMI8226. Combination of Matrine and ATO in vitro can significantly increase
the inhibition effect on the proliferation of RPMI8226, with a synergistic cytotoxicity when the domino effect of
the combination above 50%. Matrine induced apoptosis of myeloma cells. |
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