| 李 徐,陈祖鹏,徐 震,黄李法.安宫牛黄丸对大鼠缺血性脑损伤后HSP70表达的影响[J].浙江中西医结合杂志,2012,22(8):595-597 |
| 安宫牛黄丸对大鼠缺血性脑损伤后HSP70表达的影响 |
| Influence of Angong Niuhuang Pills on Heat Shock Protein70 Expression in Rats with Cerebral IschemiaInjury |
| 投稿时间：2012-02-23 |
| DOI： |
| 中文关键词: 大鼠 脑缺血 安宫牛黄丸 热休克蛋白70 |
| 英文关键词:Angong Niuhuang pills cerebral ischemia HSP70 |
| 基金项目: |
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| 中文摘要: |
| 目的： 探讨安宫牛黄丸对大鼠脑缺血后脑组织内热休克蛋白70 （HSP70） 表达的影响及意
义。方法： 线栓法制备大鼠局灶性脑缺血模型， 将55只SD大鼠随机分为假手术组 （5只）、 缺血对照
组 （25只）、 安宫牛黄丸治疗组 （25只）， 缺血组和治疗组再各分为12h、 24h、 3d、 7d、 14d共5个亚组，
每个亚组5只， 治疗组大鼠给予安宫牛黄丸0.5g/2mL灌服， 1天1次， 直到各亚组时间点； 对照组及
假手术组同时予等量生理盐水灌服。观察各组大鼠脑梗死体积、 组织形态学变化， 并免疫组化方
法检测HSP70蛋白含量。结果： 安宫牛黄丸治疗组较缺血组梗死灶体积明显减少 （P<0.05）， 缺血
组HSP70阳性细胞逐渐增多， 于3天达最高峰， 然后逐渐降低。在缺血再灌注3天后开始， 安宫牛
黄丸治疗组HSP70阳性细胞较缺血对照组明显增多 （P<0.05）。显微镜下见HSP70细胞主要表达
于缺血半暗带区。结论： 安宫牛黄丸治疗对大鼠局灶性脑缺血再灌注损伤有保护作用， 其作用机
制可能与增加HSP70表达有关。 |
| 英文摘要: |
| Objective: To investigate the effectiveness of Angong Niuhuang Pill(ANGHP) on heat shock protein70
(HSP70) expression in rats with cerebral ischemia injury. Methods: The models with focal cerebral ischemia in
rats were established by the suture occluded method. Fifty-five SD rats were randomly divided into 3 groups:
sham group(5), ischemia group(25),and AGNHP group(25). The ischemia group and AGNHP group were further di?
vided into 5 subgroups:12h,24h,3d,7d, and 14d(5 in each subgroup). In the AGNHP group,rats were given gavage
of AGNHP 0.5g/2ml once a day until the ending time of each subgroup,whereas the sham group and the isch?
emia group were given NS. The infarct volume of the rat brains in each group was determined,the neurons were
observed under microscopy,and the expression of HSP70 was detected by immunohistochemistry. Results: The in?
farct volume of the AGNHP group was reduced than that of the ischemia group with a significant difference(P<
0.05). The HSP70 expression in the ischemia group was gradually increased to a peak in 3 days, and then turn
to decrease. Starting from the 3rd day, the HSP70 expressions in the AGNHP groups was increased significantly
as compared with that in the ischemia group(P<0.05). The HSP70 cells mainly expressed in the ischemic penum?
bra district. Conclusion: AGNHP could protect brain cell in rats with cerebral ischemia by increasing the expres?
sion of HSP70. |
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