| 王 静,陈 眉.补脾益气对脾虚证小鼠学习记忆的影响[J].浙江中西医结合杂志,2012,22(7):511-516 |
| 补脾益气对脾虚证小鼠学习记忆的影响 |
| Influence of Invigorating Spleen and Replenishing Qi Herbals on Learning and Memory of Mice withSpleen Deficiency |
| 投稿时间：2011-10-25 |
| DOI： |
| 中文关键词: 小鼠 脾虚证 学习记忆 神经元型一氧化氮合酶 |
| 英文关键词:mice spleen deficiency learning and memory neuronal nitric oxide synthase |
| 基金项目: |
|
| 摘要点击次数: 1211 |
| 全文下载次数: 2 |
| 中文摘要: |
| 目的： 观察补脾益气中药对脾虚症小鼠学习记忆的影响， 并探讨其作用机制。方法： 采用
利血平皮下注射建立脾虚小鼠模型， 补脾益气中药治疗后观察小鼠的行为学改变， 采用免疫组化
法检测小鼠脑内神经元型一氧化氮合酶 （nNOS） 蛋白表达的变化。结果： 脾虚证小鼠学习能力、 记
忆能力均下降； 海马CA1区nNOS表达减少而大脑皮层表达增加。补脾益气中药能改善脾虚小鼠
的学习能力， 但对记忆能力影响不大； 中药能调节小鼠海马CA1区及大脑皮层nNOS蛋白表达阳性
细胞数 （P<0.05， P<0.01）。结论： 补脾益气中药可能通过调节nNOS蛋白来改善学习记忆能力。 |
| 英文摘要: |
| Objective: To observe the effect of invigorating spleen and replenishing qi herbals on learning and
memory functions of mice with spleen deficiency and explore the underlying mechanism. Methods: The spleen-defi?
ciency model was applied by injecting reserpine under subcutaneous dorsum. Alteration of behaviors after herbal
treatment was observed in mice. The genetic expression of neuronal nitric oxide synthase (nNOS) in the brain tis?
sue was determined by immunohistochemistry. Results: Compared with the normal group, the ability of learning
and memory significantly reduced in the model group. After herbal treatment, the ability of learning was im?
proved but the memory function did not; the expression of nNOS decreased in CA1 of hippocampi area but in?
creased in cortex (P<0.05,P<0.01). Conclusion: Invigorating spleen and replenishing qi herbals can improve the
function of learning by regulating the content of nNOS in the brain. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
