| 董晓巧,俞文华,张祖勇,朱 强,车志豪,陈 锋,杜 权,王 昊,陈 军.氧化苦参碱对脑外伤大鼠脑组织IL-1β、 TNF-α
和IL-6水平的影响[J].浙江中西医结合杂志,2012,22(7):508-516 |
| 氧化苦参碱对脑外伤大鼠脑组织IL-1β、 TNF-α
和IL-6水平的影响 |
| Suppression of Oxymatrine on the Levels of Interleukin-1beta, Tumor Necrosis Factor-beta, and Interleu?kin-6 in Traumatic Rat Brain Tissue |
| 投稿时间：2011-12-16 |
| DOI： |
| 中文关键词: 大鼠 脑外伤 氧化苦参碱 IL-1β TNF-α IL-6 |
| 英文关键词:rats traumatic brain injury oxymatrine interleukin-1beta tumor necrosis factor-beta interleu?
kin-6 |
| 基金项目:浙江省中医药科技计划项目 （No.2010ZA101） |
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| 中文摘要: |
| 目的： 观察氧化苦参碱对脑外伤大鼠脑组织白介素-1β（IL-1β）、 肿瘤坏死因子-α
（TNF-α） 和白介素-6 （IL-6） 水平的影响。方法： 选用健康雄性Wistar大鼠140只， 随机分成假手术
组、 脑外伤组、 氧化苦参碱大剂量组及小剂量组， 每组35只， 利用改进的Feeney自由落体损伤装置
制作脑外伤动物模型， 分别于脑外伤模型形成后给予0.9%氯化钠注射液1mL及不同剂量氧化苦参
碱 （60和120mg/kg） 腹腔注射， 1天1次， 共5天， 在脑外伤后2h、 6h、 12h、 1d、 2d、 3d和5d断头获取脑
组织， 利用酶联免疫吸附试验测定脑组织IL-1β、 TNF-α和IL-6水平。结果： 脑外伤后2h、 6h、 12h、
1d、 2d、 3d和5d， 氧化苦参碱小剂量组大鼠脑组织IL-1β、 TNF-α和IL-6水平与脑外伤组比较， 差异
无统计学意义 （P>0.05）； 脑外伤后12h、 1d、 2d、 3d和5d， 氧化苦参碱大剂量组大鼠脑组织IL-1β、
TNF-α和IL-6水平较脑外伤组显著减少 （P<0.05）， 脑外伤后2h和6h， 氧化苦参碱大剂量组大鼠脑
组织IL-1β、 TNF-α和IL-6水平与脑外伤组比较差异无统计学意义 （P>0.05）。结论： 氧化苦参碱可
抑制脑外伤大鼠脑组织的炎症反应。 |
| 英文摘要: |
| Objective: To investigate the effect of oxymatrine on interleukin-1beta（IL-1β）， tumor necrosis fac?
tor-beta （TNF-α） , and interleukin-6（IL-6）levels in traumatic rat brain tissue. Methods: A total of 140 male
Wistar rats were randomly divided into sham operation group， brain trauma group， and low and high dose treat?
ment group. The rat model of traumatic brain injury was induced by a modi?cation of Feeney’ s weight-drop mod?
el. Oxymatrine was given to rats once a day in the low and high treatment（60 and 120 mg/kg）groups via in?
traperitoneal injection after trauma for 5 days. The rats in the brain trauma and sham operation groups received
an intraperitoneal administration of 1 mL of saline after trauma or procedures. Animals were sacrificed by decapi?
tation at hour 2， 6 and 12， as well as day 1， 2， 3， and 5 after trauma. Brains were removed and IL-1β， TNF-α，
and IL-6 levels in rat brain tissues were measured using enzyme linked immunosorbent assay. Results: At hour
2， 6 and 12， as well as day 1， 2， 3， and 5 after trauma, the differences between IL-1β， TNF-α， and IL-6 levels
of brain tissues in the low dose treatment and brain trauma groups were not statistically significant （P>0.05） .
At hour 12， as well as day 1， 2， 3， and 5 after trauma， IL-1β， TNF-α， and IL-6 levels of brain tissues in the
high dose treatment group were significantly lower than those in the brain trauma group （P<0.05） ,but at hour 2
and 6， these differences were not significant（P>0.05） . Conclusion: Oxymatrine may suppress inflammatory reac?
tions in traumatic rat brain tissues. |
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