| 周华妙,郭 勇.寒凝血瘀对结肠癌肺转移模型小鼠肺组织VEGF、 MMP-2表达的影响[J].浙江中西医结合杂志,2012,22(6):429-432 |
| 寒凝血瘀对结肠癌肺转移模型小鼠肺组织VEGF、 MMP-2表达的影响 |
| Effect of Blood Stasis Due to Cold on the Expression of Lung Tissue VEGF and MMP-2 in Mice withMetastatic Lung Lesions from Colon Cancer |
| 投稿时间：2011-12-15 |
| DOI： |
| 中文关键词: 小鼠 结肠癌 血行转移 寒凝血瘀 基质金属蛋白酶-2 血管内皮生长因子 |
| 英文关键词:mice colon cancer hematogenous metastasis syndrome of blood stasis due to cold matrix metal?
loproteinase 2 vascular endothelial growth factor |
| 基金项目: |
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| 中文摘要: |
| 目的： 观察寒凝血瘀状态下Balb/c小鼠C26人工血行肺转移组织血管内皮生长因子
（VEGF）、 基质金属蛋白酶-2 （MMP-2） 表达。方法： 将小鼠随机分为空白对照组、 寒凝血瘀组、 单纯
荷瘤组、 复合造模组， 分别进行造模， 通过免疫组化、 Western Blot方法检测肺转移灶中VEGF、
MMP-2的表达。结果： 复合造模组小鼠基质金属蛋白酶-2 （MMP-2） 表达高于单纯荷瘤组， 差异有
统计学意义 （P<0.05）， 复合造模组小鼠血管内皮生长因子 （VEGF） 表达较单纯荷瘤组小鼠有增高
趋势， 差异无统计学意义 （P>0.05）。结论： 寒凝血瘀状态可能通过促使结肠癌小鼠肿瘤组织VEGF
和MMP-2表达的上调， 促使小鼠C26结肠癌血行肺转移， 促进疾病进展。 |
| 英文摘要: |
| Objective： To investigate the role of blood stasis caused by cold in the expression of vascular endothe?
lial growth factor(VEGF)and matrix metalloproteinase 2(MMP-2) in lung tissues of Balb/c mice with metastatic
lung cancer. Methods: Twenty-four Balb/c mice were equally randomized into 4 groups: normal control group,
blood stasis group, colon cancer group, and blood stasis-cancer group. Models were established in other 3
groups except the normal controls. The expression of VEGF was detected with immunohistochemistry and MMP-2
with Western blotting. Results: The expression of MMP-2 in the blood stasis-cancer group was significantly high?
er than that in the cancer group(P<0.05). The expression of VEGF in the blood stasis-cancer group was higher
than that in the cancer group without a significant difference(P>0.05). Conclusion: The state of blood stasis
caused by cold may prompt the hematogenous metastasis of colon cancer to the lung by upregulating the levels
of VEGF and MMP-2 in tumor tissues. |
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