| 程军,张雯,程擎天,羊淑敏.系统性红斑狼疮小鼠肾组织γδT 细胞TGF-β1 表达研究[J].浙江中西医结合杂志,2012,22(2):89-91 |
| 系统性红斑狼疮小鼠肾组织γδT 细胞TGF-β1 表达研究 |
| Production of TGF -β1 by γδT Cells in the Kidneys of MRL/lpr Mice with Systemic Lupus Erythematosus |
| 投稿时间：2011-08-31 |
| DOI： |
| 中文关键词: 小鼠 系统性红斑狼疮 TCRγδ细胞 转化生长因子β1 |
| 英文关键词:IgA nephropathy TCRγδ cell transforming growth factor-β1 |
| 基金项目:杭州市科技局资助项目（No.2006533Q18） |
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| 中文摘要: |
| 目的：研究系统性红斑狼疮（SLE）MRL/lpr 小鼠肾组织γδT 淋巴细胞分泌细胞因子TGF－
β1 的功能。方法：流式细胞仪分析MRL/lpr 小鼠外周血、肾组织γδT 淋巴细胞比例，并体外选择性
扩增γδT 细胞，在体外培养条件下，测定培养体系中细胞因子TGF－β1 的表达。结果：MRL/lpr 小鼠
外周血、肾组织中γδT 淋巴细胞比例升高，肾组织中γδT 淋巴细胞能异常分泌TGF－β1 细胞因子。
结论：肾组织γδT 细胞可能通过分泌TGF－β1 在狼疮性肾炎发病中起着免疫调节的保护性功能。 |
| 英文摘要: |
| Objective：To investigate the production of TGF-β1 by stimulated γδT cells in the kidneys from MRL/lpr mice
with systemic lupus erythematosus. Methods：The proportion of γδT cells in peripheral blood and renal tissues in MRL/lpr
mice were measured by flow cytometry. Then γδT cells was expanded in vitro to detemine the level of active TGF-β1 in
supernatants by ELISA. Results：The proportion ofγδ T cells in peripheral blood mononuclear cells and the renal tissues
from MRL/Ipr mice was significantly higher than those in the controls. The level of TGF-β1 produced by activated γδT
cells in renal tissues from MRL/Ipr mice was significantly higher than that from the controls.Conclusions：γδT cells may
play an important role in the pathogenesis of systemic lupus erythematosus via production of TGF-β1. |
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