| 卢莉莉,程泾,程蕾,黄鹏.益肾导痰调冲汤对多囊卵巢综合征大鼠瘦素及其受体和IGF-IR表达的影响[J].浙江中西医结合杂志,2012,22(1):11-14 |
| 益肾导痰调冲汤对多囊卵巢综合征大鼠瘦素及其受体和IGF-IR表达的影响 |
| Effect of Yishendaotantiaochong Decoction on Leptin and Leptin Receptor and Insulin-like Growth Factor I Receptorin Polycystic Ovary Syndrome Rats |
| 投稿时间：2011-03-10 |
| DOI： |
| 中文关键词: PCOS 大鼠 瘦素 瘦素受体 胰岛素样生长因子受体 益肾导痰调冲汤 |
| 英文关键词:polycystic ovary syndrome rats leptin OB-R insulin-like growth factor I receptor Yishendaotantiaochong
decoction |
| 基金项目:浙江省温州市卫生科技发展计划（No.S08177）；温州市鹿城区科技计划项目（No. S0070111） |
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| 中文摘要: |
| 目的：观察益肾导痰调冲汤对多囊卵巢综合征（PCOS）大鼠瘦素及其受体和胰岛素样生长
因子I 受体（IGF-IR）表达的影响。方法：SD 大鼠，采用“T＋HCG”造模法建立PCOS 大鼠模型。随机
分为正常对照组、空白模型组、益肾导痰调冲汤等效剂量组（以下简称中药组）、西药达英－35 组、达
英加中药组、桂枝茯苓丸组各10 只。观察卵巢组织形态学改变；放射免疫法测定血清内分泌水平；
ELISA 法测定瘦素（Lep）；免疫组化法测定卵巢组织瘦素受体（OB-R）及IGF-IR 表达。结果：益肾导
痰调冲汤可改善PCOS 大鼠卵巢组织结构， 缩小卵巢体积， 减少囊状卵泡及闭锁卵泡数目。降低
LH、LH/FSH、T、Lep，增高E2 水平，降低OB-R、IGF-IR 阳性表达（P＜0.05 或P＜0.01）。结论：益肾导
痰调冲汤可改善PCOS 大鼠lep 与OB-R 的结合障碍，调节OB-R、IGF-IR 的生物利用度，从而改
善PCOS 大鼠的高雄激素血症及排卵障碍。 |
| 英文摘要: |
| Objective： To observe the effect of Yishendaotantiaochong decoction（YSDTTCD）on the expression of rat leptin
and its receptor (OB-R) and insulin-like growth factor I receptor(IGF-IR) with polycystic ovary syndrome（PCOS）.
Methods: Rat models with PCOS were established by hypodermic injection with T and HCG. SD rats were randomly divided
into 6 groups: normal control,model matched control,YSDTTCD-treated group t,Diane-35 pill-treated group, YSDTTCD
and Diane-35-treated group, and Guizhifuling pill-treated group. The ovaries were harvested from the rats for histomorphological
observation. The levels of serum endocrine index were determined by radio-immunity. Serum leptin was determined
by ELISA. OB-R and IGF-IR in the tissues of ovaries was assessed with immunohistochemistry method. Results:
By treatment with YSDTTCD the ovarian volume and the number of vesicular follicle and atretic follicle in ovarian tissue
were decreased, the levels of LH, LH/FSH,T,and leptin were downregulated, and the serum level of E2 was increased. The
expression of OB-R and IGF-IR were reduced in the tissue of ovaries(P<0.05 or P<0.01). Conclusion：YSDTTCD may interrupt
the combination of leptin and OB-R in PCOS rats and regulate the biological availability of OB-R and IGF-IR in
ovaries, to improve high serum-T and ovulation barrier. |
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